The 5-Amino-1MQ dosage sold across clinic and vendor pages has no human study behind it. The published record for this compound is two mouse experiments. A 2018 study injected 20 milligrams per kilogram three times a day for 11 days into obese male mice. A 2022 study injected 32 milligrams per kilogram of active ingredient once a day for about seven weeks. No trial has given the compound to a person.
No human 5-amino-1MQ dosage exists. The animal numbers cannot cross the species line by arithmetic, and the vial a buyer receives may not even state which salt weight the milligram on the label refers to.
A reader who types a dose into a search engine wants a number to check against a vial. The record offers a mouse number, a salt weight and a regulatory silence.
The Molecule In The Vial Is Not A Peptide
5-amino-1-methylquinolinium is a quinolinium cation: two fused rings with a permanent positive charge, formula C10H11N2+, molecular weight 159.21 (PubChem CID 950107, read October 30, 2026). Sellers ship it in the same lyophilized vials used for peptides, and the name begins with “5-amino”, which reads like an amino acid. There is no amino acid chain in it. It is a small synthetic molecule that happens to be sold beside BPC-157 and CJC-1295.
The molecule blocks an enzyme. Nicotinamide N-methyltransferase, NNMT, converts nicotinamide into 1-methylnicotinamide and consumes methyl groups the cell would otherwise spend on SAM. Neelakantan and colleagues measured the effect in fat cells: less 1-MNA, more NAD+ and more SAM inside the cell (Biochemical Pharmacology, 2018). Our compound status pages track where each of these molecules sits in the federal record.
An outcome in a person requires a trial in people, and that trial does not exist.
The Only 5-Amino-1MQ Dosage Given To A Living Animal
The 2018 paper is the dosing record. Neelakantan and colleagues at the University of Texas Medical Branch took 17-week-old male C57Bl/6 mice that had eaten a diet drawing 45 percent of its energy from fat for 15 weeks, then treated them for 11 days with 20 milligrams per kilogram of 5-amino-1MQ under the skin three times a day. Nine mice received the drug and nine received saline (Biochemical Pharmacology, January 2018).
The result, from the paper’s own figures:
- Saline mice gained 0.6 grams across the 11 days, about 1.4 percent of body weight.
- Treated mice lost 2.0 grams, about 5.1 percent.
- Epididymal fat pad mass fell about 35 percent and fat cells shrank by more than 30 percent.
- Total cholesterol ran about 30 percent lower.
- Food intake did not differ: 28.1 grams in the saline group against 26.2 grams in the treated group.
The authors reported no observable adverse effects at that dose across those 11 days. In cultured fat cells the compound lowered 1-MNA with a half maximal concentration of 2.3 micromolar, and the enzyme assay put its potency at 1.2 micromolar. In a cell viability test at the top of the range, 600 micromolar, the compound killed about 40 percent of the cells. That detail sets the boundary on the phrase “no adverse effects”: a mouse, a dose, 11 days.
A Second Study, A Salt, And Why The Milligrams Move
In 2022 Dimet-Wiley and colleagues published a mouse study in Scientific Reports with a different endpoint, the gut microbiome, and a different dose: 32 milligrams per kilogram of active ingredient injected once a day for about seven weeks, in mice moved from a 45 percent fat Western diet onto a 10 percent fat diet. Body weight and fat mass dropped to the range of age-matched lean animals (Scientific Reports, January 10, 2022).
Read the methods sentence closely, because it explains half of the confusion on the dosage pages. The paper records the injection as “4 mg 5A-1MQ monochloride salt/mL sterile saline dosed at 10 mL/kg body weight” and calls that 32 milligrams per kilogram of active pharmaceutical ingredient. Forty milligrams of the salt delivers 32 milligrams of the molecule. The chloride counterion makes up the difference.
The counterion matters at the vial. The cation weighs 159.21 grams per mole. The chloride salt weighs 194.66 and the iodide form, recorded under CAS number 42464-96-0, weighs 286.11 (PubChem CID 66522933 and CID 176507677, read October 30, 2026). A 10 milligram iodide vial carries about 5.6 milligrams of the molecule. A 10 milligram chloride vial carries about 8.2. A label that prints ten without naming the form has told a buyer less than half of what it appears to say.
Why No 5-Amino-1MQ Dosage Chart Can Be Written From Mice
A mouse milligram per kilogram does not become a human milligram by dividing by a constant. Four gaps sit between the two studies above and a person holding a vial.
The first is duration. Eleven days and seven weeks are the entire safety record. Neither study ran long enough to see what a compound that alters NAD+ and SAM flux does over months, and a clinic protocol runs for months.
The second is species handling. The 2022 authors note that they injected the compound because 5-amino-1MQ “specifically has poor oral bioavailability in mice, a phenomenon that does not occur in rats”. Oral absorption differs between two rodents. It has not been measured in humans at all, which leaves the capsules sold online with no pharmacokinetic footing in any species.
The third is the molecule’s charge. A permanent cation crosses membranes through transporters rather than by dissolving through them, and the 2018 paper points to organic cation transporters as the likely route while noting that the transport mechanism remains unstudied. Blood levels in people are unmeasured, so no one knows what a given milligram reaches.
The fourth is the product. Compounded vials carry no FDA review of identity, purity, potency or sterility. A published mouse dose assumes a characterized chemical.
What The Regulators Have Not Said
DailyMed, the federal label database, holds no entry for this compound under its short name or under NNMT (searched October 30, 2026). ClinicalTrials.gov lists no registered study of it on the same date. FDA’s Pharmacy Compounding Advisory Committee briefing document of December 4, 2024 runs 72 pages on CJC-1295 and ipamorelin, and the compound name, NNMT and quinolinium appear on none of them. We read all 72 pages on October 30, 2026.
No regulator has examined this molecule for use in people, which is a different finding from a regulator examining it and finding it safe.
We looked at the live results for this query on October 30, 2026. The first page was calculator pages and vendor guides, and not one of them cited a human study.
What We Would Ask Before Buying A 5-Amino-1MQ Vial
The questions work in any order and each one has a document behind it.
Which salt does this label describe, and what is the milligram figure for the cation alone? Did any human receive it in a monitored study, and where is that study? What is the blood level expected at this dose, measured in a person? What monitoring covers the months a protocol runs? Who holds the license that makes this legal to dispense where the buyer lives?
Doses vary by product, route and person. The decision belongs to a prescriber who has examined the patient and can order labs. Our clinic directory lists clinics by city and each license is worth checking with the state board before booking. Our piece on MOTS-c dosage applies the same test to another metabolic compound sold on thin human data, and the TB-500 dosage page does it for a peptide with a single human study.
Frequently asked questions
What is the standard 5-amino-1MQ dosage?
No standard human dose exists. The two published animal studies used 20 milligrams per kilogram three times a day for 11 days and 32 milligrams per kilogram once a day for about seven weeks. A dose for one person requires a prescriber’s assessment and has no published basis.
Has 5-amino-1MQ been tested in humans?
No. A ClinicalTrials.gov search on October 30, 2026 returned no registered study of the compound, and no published paper reports it given to a person. The published record consists of laboratory work on cells and two mouse experiments.
Why does the milligram change depending on the salt?
Because a salt milligram includes the counterion. The active cation weighs 159.21 grams per mole, the chloride salt 194.66 and the iodide salt 286.11. Ten milligrams of the iodide holds about 5.6 milligrams of the molecule, while ten milligrams of the chloride holds about 8.2.
Is 5-amino-1MQ a peptide?
No. It is a quinolinium small molecule with a permanent positive charge, sold in peptide vials because the same shops carry both. It has no amino acid chain and no approved drug containing it.
How much weight did the mice lose?
The 2018 study ran 11 days and the treated group lost 2.0 grams of body weight against a 0.6 gram gain in the saline group, with fat pad mass down about 35 percent and no change in food intake. A mouse result of that size says nothing about the size of a human result.
Where do the primary documents sit?
The 2018 Biochemical Pharmacology paper and the 2022 Scientific Reports paper are linked below, with the FDA briefing document, the PubChem records and the two database searches we ran on October 30, 2026. Start with the 2022 Scientific Reports methods section, where the mouse dose and the salt form appear in one sentence.
Sources:
- Neelakantan H, Vance V, Wetzel MD, Leo Wang HY, McHardy SF, Finnerty CC, Hommel JD, Watowich SJ. Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse high fat diet-induced obesity in mice, Biochemical Pharmacology 2018;147:141-152, doi 10.1016/j.bcp.2017.11.007.
- Dimet-Wiley A, Wu Q, Wiley JT, Eswar A, Neelakantan H, Savidge T, Watowich S. Reduced calorie diet combined with NNMT inhibition establishes a distinct microbiome in DIO mice, Scientific Reports 2022;12:484, doi 10.1038/s41598-021-03670-5, January 10, 2022.
- FDA, Pharmacy Compounding Advisory Committee briefing document, December 4, 2024, 72 pages, read October 30, 2026.
- PubChem, 5-amino-1-methylquinolinium, CID 950107, molecular weight 159.21, read October 30, 2026.
- PubChem, 5-amino-1-methylquinolinium iodide, CID 66522933, molecular weight 286.11, CAS 42464-96-0, read October 30, 2026.
- PubChem, 5-amino-1-methylquinolinium chloride, CID 176507677, molecular weight 194.66, read October 30, 2026.
- DailyMed search for 5-amino-1MQ, run October 30, 2026, no label returned.
- ClinicalTrials.gov search for 5-amino-1MQ, run October 30, 2026, no registered studies returned.
This article is reporting on published papers, chemical records and database searches for a directory of clinics and patients. It is not medical advice, and it does not tell anyone what to take.